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The substrate Option was then saved at −20°C right until the ammonium and nitrate contents ended up analyzed Using the San++ Steady-Move Analysator (Skalar Analytic GmbH, Erkelenz, Germany). Nitrate perseverance was determined by a response with hydrazinium sulfate, sulfanilamide and N-(one-naphthyl) ethylenediamine dihydrochloride which ends up